Nuclear magnetic resonance analysis of carotenoids from the burgundy plumage of the Pompadour Cotinga (Xipholena punicea).

TitleNuclear magnetic resonance analysis of carotenoids from the burgundy plumage of the Pompadour Cotinga (Xipholena punicea).
Publication TypeJournal Article
Year of Publication2013
AuthorsLaFountain AM, Pacheco C, Prum RO, Frank HA
JournalArchives of Biochemistry and Biophysics
Volume539
Issue2
Pagination133-41
Date Published2013 Nov 15
ISSN1096-0384
KeywordsAnimals, Carotenoids, Chromatography, High Pressure Liquid, Feathers, Male, Mass Spectrometry, Nuclear Magnetic Resonance, Biomolecular, Passeriformes
Abstract

Previous analysis of carotenoids extracted from the burgundy plumage of the Pompadour Cotinga (Xipholena punicea) revealed six novel keto-carotenoid pigments with methoxyl groups in the C3-position of one or both β-rings. High performance liquid chromatography (HPLC), mass spectrometry, chemical analysis and, in some instances (1)H NMR spectroscopy were employed to determine the structures of the molecules. Further analysis by NMR was precluded due to lack of material. The recent acquisition of multiple feathers from X. punicea specimens has made it possible to complete this work using correlated homonuclear spectroscopy (COSY), nuclear overhauser effect spectroscopy (NOESY) and (1)H NMR. These new data conclusively confirm the structures of the six methoxy-carotenoids suggested by the earlier work. In addition, the resonance positions of the protons from the novel 3-methoxy-4-keto-β-ring and 2,3-didehydro-3-methoxy-4-keto-β-ring moieties are reported here for the first time.

DOI10.1016/j.abb.2013.08.012
Custom 1

http://www.ncbi.nlm.nih.gov/pubmed/23999492?dopt=Abstract

Alternate JournalArch. Biochem. Biophys.
PubMed ID23999492